Figure 2. IRF8 regulates MMP3 promoter activity in conjunction with PU.1.

A. Chromatin immunoprecipitation (ChIP) followed by RT-PCR on the indicated cell lines to determine binding of IRF8 and PU.1 to the putative consensus motif of the mouse MMP3 promoter (−1137 base pairs upstream of the TSS). Data represent one of two independent experiments. B. ChIP followed by quantitative PCR on the indicated cell line to determine enrichment of IRF8 or PU.1 at the putative consensus motif. C. Transfection assays using the CMS4 cell line to measure MMP3 promoter activity in the presence of cDNA encoding full-length IRF8, PU.1 cDNA or both (upper panel) vs. the corresponding empty vector control plasmids (lower panel). D. EMSA using CMS4-SC lysates after incubation with or without the indicated ³²P-labeled oligonucleotide probe. Data are representative of two separate experiments.