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. 2015 May 8;6(17):15164–15179. doi: 10.18632/oncotarget.3897

Figure 8. Tumor-derived MMP3 modulates invasive capacity and MMP9 activity in vitro.

Figure 8

A. Invasion capacity of the indicated tumor cell line (SC vs. MMP3lo), as determined by basement membrane penetration in two-dimensional invasion chamber assays (first bar of each pair set at 1). B. Assays in A performed in the absence or presence of the pan MMP inhibitor GM-6001 for the CMS4.met.sel model. C&D. MMP9 activity, as assessed by zymography. Upper, representative gels for the indicated tumor cell line pairs (SC vs. knockdown), which shows in situ digestion of gelatin within the gel matrix. Lower, quantification for each cell line; data obtained by densitometry of each band. Each value was then divided by the respective sample-volume to yield a measurement of activity per μl of cell-free supernatant. The values for each cell line were then pooled across three separate experiments and reported as mean ± SEM.