Figure 1. PLX4032 increases RHOB expression in BRAFV600E melanoma cells.
A and B. RT-qPCR analysis of RHOGTPase transcripts in WM266-4 and A375 cells treated with 1 μM PLX4032 for 48 h. C and D. Western blotting of RHOB, p-ERK and ERK in BRAF-mutant melanoma cells treated with 1 μM PLX4032 for 48 h. E and F. Western blotting of the indicated proteins in WM266-4 cells treated with the indicated PLX4032 concentrations for 48 h (E) and for the indicated times with 1 μM PLX4032 (F). G. WM266-4 cells were treated with 1 μM PLX4032 for 48 h, then washed and cultured in PLX4032-free medium for the indicated times. RHOB, RHOA, RHOC, p-ERK and ERK were analyzed by Western blotting. Actin was the loading control.