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. 2015 May 7;6(17):15250–15264. doi: 10.18632/oncotarget.3888

Figure 3. RHOB is induced by PLX4032 through transcriptional activation in an iCCAAT and c-Jun dependent pathway.

Figure 3

A. After a 48 h incubation of WM266-4 cells with PLX4032 (1 μM), RHOB mRNA half-life was measured by RT-qPCR using the transcription inhibitor actinomycin D (5 μg/mL) and normalized to the long half-life of actin mRNA (one representative experiment). B-D. Luciferase assays in WM266-4 cells co-transfected with RHOB promoter-firefly luciferase constructs and pRL-CMV before treatment with PLX4032 (2 μM, 24 h in B and C; 1 μM, 48 h in D). E and F. Western blotting of p-c-Jun, c-Jun, RHOB, p-ERK and ERK in WM266- 4 cells treated with 1 μM PLX4032 for the indicated times (E) or transfected with c-Jun-targeting or non-targeting (si-Ctl) siRNAs before treatment with 1 μM PLX4032 for 48 h F. Actin was the loading control.