Sequences of real-time PCR primers.
| TARGET | sequences | Position (RefSeq) on mRNA and amplified variants (V) |
|---|---|---|
| TP73 | F: GGACGTACTCCCCGCTCTTGA R: TCCGCTTTCTTGTAAACAGGCATG |
All 13 variants are theoretically amplified$ |
| PPIA | F: GAGCACTGGAGAGAAAGGATTTGGTT R: CGTGTGAAGTCA-CCACCCTGACA |
200–289 |
| TBP | F: TTGACCTAAAGACCATTGCACTTCGT R: TTACCG-CAGCAAACCGCTTG |
610–678 (V2) |
| YWHAZ | F: CCCTCAAACCTTGCTTCTAGGAGA R: TCATATCGCTCAGCCTGCTCG |
168–214 (V4) |
$
For clarity reason the nucleotide positions on each TP73 amplicon are not indicated.