Figure 1. HTM cells exhibit hallmarks of senescence at advanced passage.

(A) HTM cells under routine culture are relatively small and elongated, and have minimal SAβGal activity. (B) After serial passaging, HTM cells assume a senescent phenotype included an enlarged, flattened morphology, and pronounced SAβGal activity. Images are of HTM728 (passages 5 and 7) and are representative of cells from other donors. Scale bars are 50 μm.