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. 2015 Apr 12;6(17):15362–15374. doi: 10.18632/oncotarget.3798

Figure 2. Intrinsic stiffness of HTM cells is increased in senescent populations.

Figure 2

(A) HTM cells from donor 517 underwent serial passaging and stiffness (Ecell) was measured each passage for both full and serum free media conditions. At terminal passage, stiffness is substantially upregulated. (B-G) HTM cells from 6 other donors (627, 621, 728, 667, 553, and 554) similarly were serially passaged to senescence, and similarly exhibited stiffening at terminal passage. Data are mean±SEM. † p < 0.05 with respect to earliest passage. (H) For all 7 donors, the average elastic modulus at terminal passage (‘Senesce.’) was normalized to average elastic modulus at the earliest passage (‘Prolif.’). For each donor and each condition, 5-7 cells were each indented 5-7 times and averaged. Data are mean±SEM. * p < 0.05; ** p < 0.01.