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. 2015 Apr 12;6(17):15362–15374. doi: 10.18632/oncotarget.3798

Figure 4. Elevated SFRP1 expression is a phenotype of HTM senescence.

Figure 4

(A) HTM cells under routine culture (HTM667; passage 6) stain positive for SFRP1 (green). DAPI is used as a nuclear counterstain. Scale bar is 50 μm. (B) After serial passaging, senescent HTM cells (HTM667; passage 10) exhibit a substantial increase in SFRP1 staining intensity. Images are of HTM667 (passages 6 and 10) and are representative of cells from other donors. Scale bars are 50 μm. (C) Quantification of the average SFRP1 staining intensity reveals a significantly increased signal in senescent cultures when compared earlier passages for full media cultures (n=3). While elevated, serum free cultures are not significant. (D) SFRP1 is elevated at the message level in serum free senescent cultures relative to earlier passages (n=3). Data are mean±SEM. * p < 0.05.