Figure 10. Cell proliferation and migration potential of exosomes and ectosomes.

(a) MTS cell proliferation assay was performed with SK-N-BE2 neuroblastoma cells treated with exosomes and ectosomes derived from SH-SY5Y cells for 24 h. As a control, untreated SK-N-BE2 cells were grown. Exosomes induced a 2-fold proliferation of SK-N-BE2 cells. On the contrary, ectosomes did not induce any significant proliferation of SK-N-BE2 cells. Error bars represent standard error of the mean, n=3, * denotes significance (p<0.05). (b) Wound healing assay of neuroblastoma cell line SK-N-BE2 is displayed. Wound was created post reaching 100% confluence, and cells were treated with either exosomes or ectosomes for 24 h. Migration was assessed at 24 h after wounding. Images were taken under the 4x objective of the light microscope. Quantification of wound closure showed that exosomes induced more migration compared to ectosomes. Error bars represent standard error of the mean, n=3, * denotes significance (p<0.05). Student's t-test was used to evaluate statistically significant differences between the values.