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. 2015 Apr 27;6(17):15397–15409. doi: 10.18632/oncotarget.3778

Figure 4. miR373-3p partially reverses TR4 function.

Figure 4

After 24 h lentivirus transfection, plasmid transient transfection by lipofectamine was performed. After 48 h, cells were seeded in cell invasion chambers with the inner wells coated with matrigel and incubated for 24–36 h. A. Invasion assay was performed in PC3 cells treated in 4 ways, scramble (scr) + negative control (NC), sh-TR4 + negative control, scr + miR-373-3p inhibitor and sh-TR4 + miR-373-3p inhibitor. Representative microphotographs of invaded cells (magnification, 100 ×). B. Quantitative analysis for the 4 PC3 groups (cell numbers were counted in six randomly chosen microscopic fields per membrane). C. Invasion assay was performed in C4–2 cells treated in 4 ways, vector, vector + OE-miR-373-3p, OE-TR4 + vector, and OE-TR4 + OE-miR-373-3p. Representative microphotographs of invaded cells (magnification, 100 ×). D. Quantitative analysis for the 4 C4–2 groups (cell numbers were counted in six randomly chosen microscopic fields per membrane).*** P < 0.001, ** P < 0.01.