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. Author manuscript; available in PMC: 2016 Sep 4.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jul 30;464(4):1314–1320. doi: 10.1016/j.bbrc.2015.07.141

Figure 3. Effect of stimulation of adenosine receptors on CD1a and CD14 cell markers expression.

Figure 3

The expression of CD1a, CD14 and CD209 cell surface markers was assessed by flow cytometry after incubation of PB monocytes with 20 ng/ml of GM-CSF and 20 ng/ml of IL-4 in the absence (Basal) or presence of 30 μM NECA for 72 hours. Representative cytofluorographic dot plots showing the percentage of CD1aposCD14neg (upper left quadrant) and CD1anegCD14pos (lower right quadrant) cells within the CD209 positive cell population in patients with bronchial asthma (A) and healthy donors (B). (C) Graphic representation of data from flow cytometry analysis of CD1anegCD14pos cells obtained from nineteen patients with bronchial asthma (BA) or twenty control subjects (Con). (D) The percentage of CD1a+CD14− cells in patients with bronchial asthma (BA, n=19) or healthy donors (Con, n=20). Data are presented as minimum, 25th percentile, 50th percentile, 75th percentile, and maximum. The Kruskal-Wallis test was significant (p <0.0001) for between group differences. Dunn’s multiple comparison post-test P values between groups are shown.