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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Clin Cancer Res. 2015 May 13;21(17):3896–3905. doi: 10.1158/1078-0432.CCR-14-3176

FIGURE 2.

FIGURE 2

Time course of radiotracer uptake and retention in etoposide- and vehicle-treated EL-4 cells. (A) Flow cytometric analysis of vehicle or drug-treated (15µM etoposide; 18h) EL-4 cells. Cell death was detected with FITC-Annexin V (λ Ex/Em = 488/535) and 7-AAD staining (λ Ex/Em = 488/647). Q1 = viable; Q2 = early apoptotic; Q3 = late apoptotic/necrotic. (B) Temporal changes in cell-associated radioactivity in 18h vehicle or etoposide-treated cells were measured with 18F-FDG, 99mTc-HYNIC-Annexin V, 18F-ML-10 and 18F-C-SNAT. Mean ± SD (n = 3–4/group). * P < 0.05, ** P < 0.01, *** P < 0.001.