Skip to main content
. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jun 12;1853(10 0 0):2560–2569. doi: 10.1016/j.bbamcr.2015.06.002

Figure 4. Regions outside of the consensus PxxPxR motifs are required for the high affinity interaction of GRB2 with SOS1.

Figure 4

A) Raw ITC tracings for the interaction of GRB2 with SOS1 WT, SOS1 Δ1234Z and SOS1 scrambled. Each trace is representative of at least 4 separate injections. B) The hydrodynamic radius of GRB2, SOS1 WT, SOS1 Δ1234Z or SOS1 scrambled, both alone and in combination, was determined using DLS. The individual values, with the mean ± 95% confidence intervals were plotted using GraphPad Prism. Statistical analysis was performed using GraphPad Prism via ANOVA analysis and the p values are shown.