Figure 3. Ventricular DNA damage response in 6-month-old female αMyHC-Cre+/− mice.
(A) mRNA expression of DNA damage response regulators, p21 and p53 (qRT-PCR). (B) DNA damage-related and pro-apoptotic protein levels (immunoblot). (C) Histochemical assessment of TUNEL+ cells in ventricular myocardium. Arrows indicate TUNEL+ cells. Error bars: SEM, N=4–6/group *P < 0.05, **P < 0.01 vs. age-matched and sex-matched WT control.