Table 1. Reported methods for A. fumigatus T-cell isolation and expansion in vitro.

Target pathogen(s)	Antigen source	Method	Cell numbers (initial/final); yield	Total time to generate T cells (days)	CD4/CD8 ratio; % antigen specific CD4 T cells	Reference
A. fumigatus	Water-soluble lysate	IFNγ capture-based isolation, stimulation with antigen-pulsed APC, feeder cells and IL-2	1.1 × 109/2 × 107; 1.8%	14	87/3; 10.3a	Tramsen et al.57
A. fumigatus	Crf1/p41 peptide	CD154 bead-based isolation, culture with feeder cells, IL-2, IL-7 and IL-15	1 × 107/3.5 × 107; 350%	14	ND; 50.6b	Stuehler et al.47
A. fumigatus	Crf1/p41 peptide	CD154 bead-based isolation, culture with feeder cells, IL-2, IL-7 and IL-15	6.86 × 107/1.86 × 107; 27%	15	ND; 40b	Khanna et al.58
A. fumigatus	Water-soluble lysate	Stimulation with antigen-pulsed APC, culture with IL-2, IL-7 and IL-15	7.4 × 106/1.94 × 108; 263%	21	94/4.5; 10.9a	Gaundar et al.31
A. fumigatus, C. albicans, R. oryzae	Water-soluble lysate	IFNγ capture based isolation, stimulation with antigen-pulsed APC, feeder cells and IL-2	1.1 × 109/5.4 × 107; 4.7%	13	98.5/0.4; 13.3a	Tramsen et al.46

Abbreviations: APC, antigen-presenting cells; ND, not determined.

^aIndicated by expression of TNFα following restimulation with antigen pulsed APC.

^bIndicated by staining with p41 tetramer antibody.