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. 2015 Sep 3;6:689. doi: 10.3389/fpls.2015.00689

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Copyright © 2015 Huang, Zeng, Xiao, Wei, Liao, Chen, Sun, Lv and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The developmental stages and the accumulation trend of the total content of four bioactive compounds in leaves of E. sagittatum. (A) Leaves at six different developmental stages were collected and their phenotypic characterizations are as follows: S1: folded young leaf with hooked petiole; S2: folded young leaf with erected petiole; S3: just expanded young leaf; S4: fully opened young leaf with one-half size mature leaf; S5: no-leathered mature leaf; S6: slightly leathered mature leaf. Bar = 1 cm. (B) The total content of four bioactive compounds (icariin, epimedin A, B, and C) in leaves at six different developmental stages from the HN3 and JX3 lines of E. sagittatum was measured by HPLC analysis. Data are presented as the mean value plus SD (standard deviation) from three biological replicates.