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. 2015 Aug 21;47(8):e178. doi: 10.1038/emm.2015.52

Figure 2.

Figure 2

Expression pattern of miR-526b and MMP1 mRNA. (a) The expression of MMP1 mRNA, normalized to the expression of GAPDH, and mature miR-526b, normalized to the expression of U6, in neonatal and adult dermal fibroblasts were analyzed using RT-qPCR. The value obtained from neonatal dermal fibroblasts was set as 1. (b) The expression of MMP1 mRNA, normalized to the expression of GAPDH, and mature miR-526b, normalized to the expression of U6, in adult dermal fibroblasts, WM115, JAR and HeLa cells were analyzed using RT-qPCR. The value of MMP1 mRNA and miR-526b miRNA expression obtained from HeLa cells were set as 1. The y axis represents the expression level in log scale. (c and d) HeLa cells and WM115 cells were treated with 5 μM 5′aza for 3 days and then treated with 1 μM TSA for 1 day. The total RNA was extracted and analyzed for mature miR-526b expression (c) and MMP1 expression (d).