Figure 4.

Effect of FAEE on leptin-induced P27 and p21 protein expression in A10 cells. (a, b) Cells were stimulated with leptin (10 ng ml⁻¹) for 3 h in the presence or absence of FAEE (1 and 10 μM). The cells were lysed and proteins were analyzed by western blotting. The β-actin was used for normalization. After densitometric quantification, data represent as mean±s.e. of six independent observation with different cell passages and on different days. *P<0.05 vs control; ^#P<0.05 vs leptin alone.