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. 2015 Aug 6;6(8):e1839. doi: 10.1038/cddis.2015.180

Figure 2.

Figure 2

3D reconstruction of evulsions in the axons of glial lamina in glaucomatous D2 mice. (a) Representative SBEM single sections (left panels) from SBEM volumes (middle panels) containing a total of 200 slices at 80-nm section thickness in the glial lamina of C57BL and glaucomatous D2 mice show striking differences in the occurrence of evulsions, protrusions and lipid droplets. The MIP of SBEM volumes are displayed with inverted image contrast (white is highest EM signal, black is no signal, right panels). Note the greater content of white osmiophilic material in the D2 sample. (b) Representative reconstructions of evulsions (green spheres) in the glial lamina of C57BL and glaucomatous D2 mice. (c) Schematic representation with the glial lamina region of immunohistochemical data collection. (d) Quantitative analysis of density, surface areas and volumes of osmiophilic materials in the glial lamina of glaucomatous D2 mice. Values are mean±S.E.M.