Figure 8.

DRP1 inhibition preserves mt morphology in the axons of glial lamina in glaucomatous D2 mice. Electron tomography generated high-resolution 3D reconstructions of mitochondria in the glial lamina of glaucomatous D2 mice transduced with AAV2-Null or AAV2-DRP1^K38A. (a) Slices (1.36-nm thick) through the middle of EM tomographic volumes of mitochondria are shown. Quantitative analysis of overexpressed DRP1^K38A showed increase of mt number but decrease of mt lengths in RGC axons of the glial lamina in glaucomatous D2 mice. However, there was no statistical difference in mt volume density. Values are mean±S.E.M. **Significant at P<0.01 and ***Significant at P<0.001 compared with glaucomatous D2 mice transduced with AAV2-Null. Scale bar: 500 nm (all panels). (b) Surface-rendered volumes of the segmented mitochondria provide information concerning shape and cristae architecture. The outer mt membrane is shown in blue (made translucent to better visualize the cristae) and cristae are in various colors