Figure 5.

PPP2R1B is a direct target of miR-587. (a) PPP2R1B expression was decreased and AKT phosphorylation and XIAP expression were increased by miR-587 expression in HCT116 and GEO cells as determined by western blot analysis. (b) The miR-587 inhibitor increased PPP2R1B expression and suppressed AKT phosphorylation and XIAP expression, determined by western blot analysis. (c) The miR-587 recognition sites, PR1 and PR2, along with their respective mutants, MR1 and MR2, are shown in the left panel. Luciferase constructs, psiCHECK2-PR1 and psiCHECK2-PR2, their mutants, psiCHECK2-MR1 and psiCHECK2-MR2, and control plasmid psiCHECK2 (Ctr) were transfected into miR-587-expressing or vector control HCT116 cells. Dual luciferase assays were performed and relative luciferase activity was determined (right panels). The data are presented as the mean±S.D. of triplicate experiments. ***P<0.001