Figure 8.

Expression of miR-587 and PPP2R1B positively and inversely correlate with chemoresistance in human colorectal cancer specimens. (a) RNA samples prepared from 9 moderately and 10 non-responding or poorly responding colorectal tumors were used to determine miR-587 expression. The data are presented as the mean±S.E. ***P<0.001. (b) IHC staining of a colorectal tumor with a PPP2R1B antibody in the absence (left) or presence (right) of a specific blocking peptide (BP) is shown, indicating the specificity of the anti-PPP2R1B antibody. Scale bars, 50 μm. (c and d) IHC staining of PPP2R1B was performed in sections prepared from colorectal tumors described above. (c) Representative images of IHC staining of PPP2R1B in each group are shown. Scale bars, 50 μm. (d) Quantification of staining intensity of PPP2R1B was performed. Error bars indicate S.E.M. of the values in each group. **P<0.01