Figure 4.

TAp63α and TAp73α can activate the TIGAR-hBS2 reporter. (a) Representative iRFP reporter assay scan of HCT116 p53^−/− cells 24 h after co-transfection with TIGAR-hBS2 iRFP reporter along with human p53, HA-tagged TAp63α or HA-tagged TAp73α. (b) Western blot analysis of HCT116 p53^−/− cells with transfected p53, HA-tagged TAp63α or HA-tagged TAp73α. (c) Quantification of iRFP reporter scans. (d) Representative iRFP reporter assay scan of HCT116 p53^−/− cells 24 h after co-transfection with TIGAR-hBS2 iRFP reporter along with TAp73α, TAp73β, TAp73γ or ΔNp73α. (e) Western blot analysis of HCT116 p53^−/− cells with transfected HA-tagged TAp73α, HA-tagged TAp73β, HA-tagged TAp73γ or HA-tagged ΔNp73α. (f) Quantification of iRFP reporter scans on human (hBS2) and mouse (mBS1 and mBS2) TIGAR promoter-binding sites. Values represent mean±S.E.M. of three independent experiments. *P<0.05, **P<0.005 compared with empty vector (EV). NT, non-transfected; NS, not significant