Figure 1.

NSC-87877 shows decreased cell proliferation in NB cell lines. (a–c) Three NB cell lines, IMR32, NB-19 and SH-SY5Y, were treated with NSC-87877 at the indicated concentrations. Cell proliferation was performed using MTT and measuring absorbance at 24 h, and at every subsequent 24 h for 5 days. Data points were compared as a fold change relative to day 1. Data were represented as a mean±S.D. P-values <0.05 for (0) versus (0.25 and 0.5) (**), <0.05 for (0) versus (0.5) (*). (d) Three NB cell lines, IMR32, NB-19 and SH-SY5Y, were seeded in six-well plates with NSC-87877, media and agar, and then grown for 2 weeks. The colonies were stained with MTT for 4 h and pictures were taken. (e) Colonies were counted and shown as mean±S.D. P-values <0.05 (*) or <0.01 (**) are indicated. Panels a to c are representative of three independent experiments. Panels d and e are representative of two independent experiments