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. 2015 Aug 6;6(8):e1836. doi: 10.1038/cddis.2015.208

Figure 5.

Figure 5

ARHI re-expression enhanced chemosensitivity to cisplatin in vitro. (a) ARHI enhanced cytotoxicity to cisplatin in short-term cell cultures. SKOv3-ARHI and Hey-ARHI cells were pre-treated with 5 μM chloroquine and 1 μg/ml DOX for 24 h. Cells were then treated with 5 μM chloroquine (CQ), 1 μg/ml DOX and 5 μM cisplatin (Cis) for additional 48 h. After treatment, cell viability was measured by SRB assay. The figure shows the combined values of three independent experiments. The columns indicate the mean, and the bars indicate the S.E. (**P<0.01 DOX− versus DOX+ *P<0.01 Cis versus no Cis). (b) ARHI enhanced cytotoxicity to cisplatin in clonogenic assays. SKOv3-ARHI, Hey-ARHI and OVCAR4-ARHI cells were grown in 6-well plates at an initial density of 2000 cells/well and allowed to settle for 24 h, and then cells were treated with DOX, chloroquine and cisplatin as described above in (a). Cell viability was measured by clonogenic assays. Data were obtained from three independent experiments. The columns indicate the mean, and the bars indicate the S.E. (**P<0.01, DOX− versus DOX+ *P<0.01 Cis versus no Cis)