Figure 2.

HK2 is required for cytoplasmic accumulation of hnRNP A1 during hypertonic stress. (a) HeLa T4+ cells were transfected with HK2-targeting (siHK2) or nontargeting control (siCTRL) siRNAs for 48 h and cell lysates were analyzed by western blot analysis. MW marker is indicated on the left. (b) HeLa T4+ cells transfected as in (a) were seeded at two different cell densities in 96-well format plates and cell confluency was monitored for 24 h by IncuCyte live cell imaging. (c) Representative confocal microscopy images of hnRNP A1 localization by immunofluorescence in sorbitol-treated cells transfected with HK2-targeting or nontargeting control siRNA. (d) Quantification of (b), where ∼250 cells per condition were counted; P=0.0031. (e) Representative confocal microscopy images of hnRNP A1 localization by immunofluorescence in sorbitol-treated cells previously treated with the HK2 inhibitor 3-BrPa. (f) Quantification of (d), where ∼2000 cells per condition were counted; P=0.0128