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. 2015 Aug 6;6(8):e1837. doi: 10.1038/cddis.2015.209

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Knockdown or inhibition of HK2 restores expression of Bcl-xL and prevents hypertonic stress-induced cell death. (a) HeLa T4+ cells were transfected with HK2-targeting (siHK2) or nontargeting control (siCTRL) siRNAs, or treated with 3-BrPa and subsequently treated with 0.4 M sorbitol. Expression of Bcl-xL, HK2, and actin (loading control) was determined by western blot analysis (left). Bcl-xL expression relative to actin was quantified by densitometry (right). MW marker is indicated on the left. (b) Cells were treated as in (a) and caspase 3 and 7 activity was determined using the CellPlayer Kinetic Caspase-3/7 reagent and the IncuCyte Zoom. The y axis represents the number of fluorescent cells per image normalized to cell confluence; n=3 (3-BrPa treatment) and n=4 (siRNA treatment)