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. 2015 Aug 13;6(8):e1856. doi: 10.1038/cddis.2015.211

Figure 2.

Figure 2

Impairment of survival and blockade of NK cell differentiation in AK2-deficient cells. CB CD34+ progenitors were transduced with shAK2 or shCont, sorted for GFP+ cells and seeded in NK cell differentiation medium for 21 days. (a) The change over time in the number of GFP+ cells during NK cell differentiation after transduction with shCont (black squares) or shAK2 (black circles). One of the three representative experiments is shown. (b) Mitochondrial depolarization was analyzed using the Mitoprobe DiIC(5) reagent 3 and then 7 days after initiation of NK cell differentiation in the shCont condition (white bars) and the shAK2 condition (black bars) (P=0.028 at D3, n=3). (c) Cell proliferation (incorporation of EdU) was analyzed 3 and 7 days after initiation of NK cell differentiation in the shCont condition (white bars) and the shAK2 condition (black bars) (n=3, P=not significant (NS)). (d) Flow cytometry analysis of GFP+ cells 21 days after initiation of NK cell differentiation in the shCont condition (dotted line) and the shAK2 condition (solid line). (e) The CD56+ cell number was evaluated 21 days after initiation of NK cell differentiation in the shCont (black squares) or shAK2 conditions (black circles) (P=0.014, n=3); *P<0.05