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. 2015 Aug 13;6(8):e1856. doi: 10.1038/cddis.2015.211

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Impaired survival and blockade of neutrophil cell differentiation in the AK2-deficient HL60 cell line. The promyelocytic cell line HL60 was transduced with shAK2 or shCont, selected with puromycin and seeded in culture with 10 μM ATRA for 4 days, in order to induce neutrophil differentiation. (a) The change over time in total cell count during neutrophil differentiation in shCont (black squares) or shAK2 (black circles) (P=0.013 on D3 and P=0.002 on D4, n=4). (b) The CD15+CD11b+ neutrophil count in differentiated HL60 cells transduced with shCont (white bar) or shAK2 (black bar) (P=0.033 on D2, P=0.008 on D3 and P=0.0003 on D4; n=4). (c) Mitochondrial depolarization was analyzed using Mitoprobe DiIC(5) reagent in HL60 cells transduced with shCont (white bar) and shAK2 (black bar) (n=4, P=NS); *P<0.05, **P<0.01, ***P<0.001