Figure 2.

NMDA injury induced Homer1a expression in vitro and in vivo. After NMDA injury in cultured cortical neurons, Homer1a mRNA was measured by qRT-PCR at different time points (a), and Homer1a protein expression was measured by WB. (b) Data are represented as mean±S.E.M. from five experiments. *P<0.05 versus 0 h group. After NMDA injection in the mouse cortex, Homer1a expression was measured by WB analysis at 6, 12 and 24 h (c), *P<0.05 versus 0 h (sham) group. Immunohistochemistry staining for Homer1a in the cortex surrounding the lesion area (d) and Homer1a-positive neurons were counted 12 h after NMDA injection (e; the sham group, n=6; NMDA group, n=9). Scale bar=100 μm. Data are represented as mean±S.E.M., *P<0.05 versus the sham group