Figure 4.

Homer1a decreased NMDA-induced Ca²⁺ influx, oxidative stress and downstream effects in vitro. After infection with different LVs, cultured cortical neurons were stimulated with NMDA. Intracellular Ca²⁺ concentrations were measured for up to 30 min (a) and the area under the curves was calculated (b). ROS production was measured by DCFH-DA fluorescent probe (c) 24 h after transfection with different LVs and NMDA injury, the phosphorylation levels of NOS (d), ERK (e) and CREB (f) were measured by WB. Data were represented as mean±S.E.M. from four experiments. *P<0.05 versus normal group; ^#P<0.05 versus NT and Vector group; **P<0.05 versus RNAi-Scr group