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. 2015 Aug 27;6(8):e1860. doi: 10.1038/cddis.2015.219

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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α-Solanine treatment caused autophagosome–lysosome fusion. A549 cells were treated with α-solanine at 10 μM for 24 h and immunostained with anti-LC3 (marker for autophagosome) and anti-LAMP2 (marker for lysosome) antibodies. Fluorescently labeled cells were analyzed under confocal microscope