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. 2015 Aug 13;6(8):e1854. doi: 10.1038/cddis.2015.226

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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GADD45β-I inhibits MKK7 activation 3 h post lesion. Western blotting and relative quantification of contralateral and ipsilateral brain samples. Animals were pre-treated with GADD45β-I (11 mg/kg 30 min before MCAO) and killed 3 and 6 h after lesion. (a) Western blotting and relative quantification of P-MKK7/MKK7 ratio 3 h after lesion. Actin was used as loading control. Data are presented as mean±S.E.M. (two-way ANOVA, Tukey's post-hoc test *P<0.05, n=3) (b) Western blotting and relative quantification of P-MKK7/MKK7 6 h after lesion. Actin was used as loading control. Data are presented as mean±S.E.M. (two-way ANOVA, Tukey's post-hoc test *P<0.05, n=3) (c) Western blotting and relative quantification of P-JNK/JNK ratio 6 h after lesion. Actin was used as loading control. Data are presented as mean±S.E.M. (two-way ANOVA, interaction >0.05, n=3) (d) Western blotting and relative quantification of P-MKK4/MKK4. Actin was used as loading control. Data are presented as mean±S.E.M. (two-way ANOVA, interaction >0.05, n=3)