Figure 2.

Functional complementation of phagocytosis with AHL. Macrophages were pretreated with 25 μM C₄-HSL and 50 μM 3O-C₁₂-HSL (C₄- C₁₂-HSL), or 0.02% DMSO as a vehicle control (Dilution control), or not-pretreated (MOI 10) for 4 h before 1-h infection with P. aeruginosa wild type or lasI-/rhlI- mutant, at MOI 10. Quantification of phagocytosis presented as the percentage of phagocytic-positive (containing ingested bacteria) cells among total macrophages. Shown are mean ± SE of four independent experiments performed at separate days from four different donors (color coded). The means ± SE are based on 100–200 cells for each condition per experiment.