Figure 3.

AQP9 protein levels are increased in macrophages infected with P. aeruginosa. (A) Macrophages were either non-infected (C) or infected with wild type P. aeruginosa or lasI-/rhlI- mutant at MOI 1, 10, and 100 for 1 h. Total cellular protein extracts were analyzed with Western blot for AQP9, 31 kDa (lower, green bands) and GAPDH, 36 kDa as a loading control (upper, red bands). The blots are from one representative of four independent experiments. (B) Densitometric analysis. Values are mean ± SE percentage of AQP9 density relative to the loading GAPDH control from four independent experiment performed at separate days from four different donors. Significant differences are indicated when ^*P < 0.05 and ^**P < 0.01, as analyzed by Student's t-test.