Figure 5.

P. aeruginosa affects cell morphology in macrophages. Macrophages were infected with wild type P. aeruginosa, the lasI-/rhlI- mutant or were non-infected (Control), stained, and imaged as in Figure 4A. (A) Quantification of the macrophage cell area. (B) Quantification of approximated macrophage length (as indicated by white arrow in Figure 4A in the direction of polarization). Columns represent means ± SE of three different experiments with three donors. Significant differences are indicated when ^*P < 0.05, ^**P < 0.01 and ^***P < 0.0001, as analyzed by two-tailed unpaired Student's t-test.