Table 1. Effect of silencing of the PxABCH1 gene on some biological parameters of the surviving larvae in P. xylostella strain DBM1Ac-S and NIL-R.
| Methods | Strain | Treatment | Pupation rate (%) | Pupal weight (mg) | Eclosion rate (%) |
|---|---|---|---|---|---|
| Microinjection | DBM1Ac-S | Buffer | 100.00 ± 0.00a | 5.12 ± 0.11a | 88.89 ± 11.11a |
| dsEGFP | 100.00 ± 0.00a | 5.03 ± 0.14a | 100.00 ± 0.00a | ||
| dsPxABCH1 | 55.56 ± 11.11b | 3.70 ± 0.20b | 0.00 ± 0.00b | ||
| NIL-R | Buffer | 100.00 ± 0.00a | 5.06 ± 0.11a | 100.00 ± 0.00a | |
| dsEGFP | 100.00 ± 0.00a | 5.10 ± 0.05a | 88.89 ± 11.11a | ||
| dsPxABCH1 | 44.44 ± 11.11b | 3.82 ± 0.09b | 0.00 ± 0.00b | ||
| Oral delivery | DBM1Ac-S | Buffer | 100.00 ± 0.00a | 5.08 ± 0.15a | 100.00 ± 0.00a |
| dsEGFP | 100.00 ± 0.00a | 5.12 ± 0.09a | 100.00 ± 0.00a | ||
| dsPxABCH1 | 44.44 ± 11.11b | 3.82 ± 0.12b | 0.00 ± 0.00b | ||
| NIL-R | Buffer | 100.00 ± 0.00a | 5.04 ± 0.12a | 88.89 ± 11.11a | |
| dsEGFP | 100.00 ± 0.00a | 5.07 ± 0.10a | 100.00 ± 0.00a | ||
| dsPxABCH1 | 55.56 ± 11.11b | 3.75 ± 0.07b | 0.00 ± 0.00b |
Values shown in the table are means and standard errors, and values with different letters in a column for each strain indicated significant differences as determined by Holm-Sidak’s test (P < 0.05; n = 3).