Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 14;5(8):e339. doi: 10.1038/bcj.2015.67

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Expression of Twist1 and its correlation with Bmi1 level in AML cell lines and primary AML samples. (a) Western blotting results of the expression levels of Twist1 protein in the whole cell lysates of two AML cell lines, THP1 and KG1a. β-Actin was used as an internal control. Representative data from three independent experiments are presented. (b) Effects of Twist1-targeted siRNA on the protein levels of Twist1 and Bmi1 in THP1 cells. Twist1-overexpressing THP1 cells were transiently transfected with either Twist1-targeted siRNA or scrambled control for 48 h, and the nuclear lysates were examined by western blotting. Histone 3 was used as a loading control. Representative data from three independent experiments are shown. (c) Positive correlation between Twist-1 and Bmi-1 gene expression in primary AML samples examined by real-time RT-PCR (r=0.5662, P<0.0001, Pearson's correlation).