Figure 2.

Effect of T-cell ligand expression levels on AMG 330-induced cytotoxicity. (a) Parental CD33⁺ ML-1 cells were transduced with PD-L1, PD-L2 or CD80 at a low MOI (0.25) or high MOI (25) to generate sublines that expressed individual T-cell ligands at different levels, as indicated by arbitrary median fluorescence units. (b) Parental cells and sublines were incubated with increasing concentrations of AMG 330 and healthy donor T-cells at an effector/target (E/T) cell ratio of 1:2.5. After 48 h, cell counts were determined and cytotoxicity was assessed with DAPI staining to quantify drug-specific cytotoxicity. Results (mean±s.e.m.) are shown from three independent experiments performed in duplicate wells.