| anti-claudin 5 (Life technologies #35-2500), 1:50 |
Brain endothelial cell marker and tight junction protein |
| anti-GFAP (Dakocytomation, Z 0334), 1:200 |
Marker of reactive astrocytes |
| anti-PDGFRß (Neuromics, GT 15065), 1:200 |
Marker of pericytes |
| anti-Ki67 (ab 15580 Abcam), 1:200 |
Cell proliferation marker |
| DAPI (Sigma, 32670), 0.5 μg/ml |
Cell nuclei staining |
| anti-Desmin (4024 Cell Signaling), 1:100 |
Pericyte marker |
| anti-pan-Laminin (ab7463, Abcam), 1:1000 |
Staining for basement membrane and laminins |
| anti-NG2 (AB5320: Merck Millipore), 1:200 |
Staining for pericyte marker NG2 |
