Table 2. Hemolytic activities of pneumolysin mutants.
| Protein | EC50 (nM) | Relative Hemolytic Activity |
|---|---|---|
| Wild type PLY | 2.6 ± 0.5 | 1.0 |
| Leu11Arg | 4.6 ± 1.6 | 0.69 ± 0.35 |
| Ser68Glu | 16.2 ± 1.6 | 0.16 ± 0.03 |
| Asn86Arg | 14.7 ± 1.5 | 0.18 ± 0.03 |
| Thr88Glu | 76 ± 20 | 0.039 ± 0.017 |
| Arg147Ala | 11 ± 1 | 0.24 ± 0.03 |
| Arg147Glu | 115 ± 2 | 0.023 ± 0.002 |
| Asp205Arg | >10000 | <0.0003 |
| Arg226Ala | 48 ± 16 | 0.064 ± 0.031 |
| Thr304Arg | 900 ± 200 | 0.0031 ± 0.0013 |
| Asp338Ala | 2.9 ± 1.1 | 1.1 ± 0.3 |
| Asp338Arg | 1.4 ± 0.4 | 0.53 ± 0.11 |
| Asn339Arg | >10000 | <0.0003 |
| Val341Arg | 2.5 ± 1.0 | 1.3 ± 0.8 |
| Arg399Ala | 15 ± 0.91 | 0.17 ± 0.02 |
| Glu434Ala | 22 ± 1 | 0.12 ± 0.02 |
| Arg437Ala | 21 ± 1 | 0.13 ± 0.01 |
| Tyr461Ala | 20 ± 1 | 0.13 ± 0.02 |
| Tyr461Phe | 1.3 ± 0.1 | 2.0 ± 0.3 |
| Arg339Ala + Glu434Ala | 48 ± 1 | 0.051 ± 0.003 |
| Glu434Ala + Tyr461Ala | 86 ± 1 | 0.030 ± 0.003 |
| Glu434A + Arg437Ala | 84 ± 4 | 0.030 ± 0.004 |
Values are mean ± error from two experiments.