Table 1.
Relationship between the frequencies of microchimeric versus autologous cells of a given immunophenotype
| Phenotype | JIIM | MD | NIC |
|---|---|---|---|
| % Autologous CD3+ cells of total autologousa | 6.5 ± 2.3 | 9.9 ± 2.5 | 4.8 ± 2.7 |
| % Microchimeric CD3+ cells of total microchimeric cellsb | 0.3 ± 0.3 | 2.8 ± 1.8 | 10 ± 10 |
| p = 0.06 (T) | p = 0.04 | p = 0.05 | |
| % Autologous CD4 cells of total autologous cells | 16.8 ± 6.9 | 12.2 ± 5.3 | 8.4 ± 6.6 |
| % Microchimeric CD4 cells of total microchimeric cells | 2.2 ± 1.4 | 7.8 ± 2.0 | 0 ± 0 |
| p = 0.03 | p = 0.23 | p = 0.12 | |
| % Autologous CD8 cells of total autologous cells | 20.4 ± 5.0 | 12.6 ± 3.2 | 5.1 ± 3.2 |
| % Microchimeric CD8 cells of total microchimeric cells | 1.1 ± 0.6 | 6.5 ± 2.3 | 0 ± 0 |
| p = 0.05 | p = 0.09 | p = 0.07 | |
| % Autologous Class II cells of total autologous cells | 21.5 ± 8.1 | 51.6 ± 5.9 | 38.0 ± 11.0 |
| % Microchimeric Class II cells of total microchimeric cells | 1.7 ± 1.1 | 0.8 ± 0.7 | 20.0 ± 13.3 |
| p = 0.04 | p < 0.001 | p = 0.02 | |
| % Autologous CD20 cells of total autologous cells | 8.5 ± 4.4 | 2.7 ± 2.5 | 0 ± 0 |
| % Microchimeric CD20 cells of total microchimeric cells | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| p = 0.08 | p = 0.3 | p = NA | |
| % Autologous CD83 cells of total autologous cells | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.2 |
| % Microchimeric CD83cells of total microchimeric cells | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| p = 0.18 | p = 0.17 | p = 0.17 | |
| % Autologous CD25 cells of total autologous cells | 16.2 ± 5.1 | 9.5 ± 3.4 | 6.4 ± 2.8 |
| % Microchimeric CD25 cells of total microchimeric cells | 0 ± 0 | 7.7 ± 2.9 | 0 ± 0 |
| p = 0.01 | p = 0.33 | p = 0.02 | |
| % Autologous CD45RA cells of total autologous cells | 2.3 ± 0.9 | 1.5 ± 0.4 | 3.2 ± 2.3 |
| % Microchimeric CD45RA cells of total microchimeric cells | 1.2 ± 1.1 | 0.9 ± 0.9 | 0 ± 0 |
| p = 0.24 | p = 0.26 | p = 0.10 | |
| % Autologous CD45RO cells of total autologous cells | 6.3 ± 2.4 | 4.1 ± 1.1 | 9.0 ± 4.4 |
| % Microchimeric CD45RO cells of total microchimeric cells | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| p = 0.03 | p = 0.003 | p = 0.06 (T) | |
| % Autologous CD123 cells of total autologous cells | 3.6 ± 1.1 | 1.9 ± 0.7 | 26.2 ± 12.2 |
| % Microchimeric CD123 cells of total microchimeric cells | 0.04 ± 0.02 | 0 ± 0 | 4.4 ± 2.3 |
| p = 0.04 | p = 0.06 (T) | p = 0.06 (T) |
Data are presented as mean ± standard error of the mean for each group
JIIM juvenile idiopathic inflammatory myopathy, MD muscular dystrophy, NIC noninflammatory controls; NA not available, T trend for significance
aTotal autologous cells consists of all phenotyped cells with XY probes
bTotal microchimeric cells consists of all cells with XX probes