Figure 3.
DA1, DAR1, and DAR2 Act Redundantly to Regulate Endoreduplication during Leaf Development.
(A) and (B) Epidermal cells in Col-0 (A) and da1-ko1 dar1-1 dar2-1 (B) fifth leaves.
(C) and (D) Palisade cells in Col-0 (C) and da1-ko1 dar1-1 dar2-1 (D) fifth leaves.
(E) Average area of palisade cells in Col-0 and da1-ko1 dar1-1 dar2-1 fifth leaves. Values are given as means ± se relative to the respective wild-type values, set at 100%. **P < 0.01 compared with the wild type (Student’s t test).
(F) Nuclear size distribution of epidermal cells in Col-0 and da1-ko1 dar1-1 dar2-1 fifth leaves.
(G) and (H) Nuclear DNA ploidy distribution of Co-0 (G) and da1-ko1 dar1-1 dar2-1 (H) fifth leaves measured over a period of 24 DAE. At 0 DAE, the fifth leaves (∼0.5 mm2) were visible. The values represent averages of three independent biological replicates.
Bars in (A) and (B) = 50 μm; bars in (C) and (D) = 100 μm.