Figure 5.

DA1, DAR1, and DAR2 Modulate the Stability of TCP14/15.

(A) to (D) A proteasome inhibitor stabilizes TCP14 and TCP15. Histochemical analysis of GUS activity of 10-d-old TCP14_pro:TCP14-GUS (A) and TCP15_pro:TCP15-GUS (B) seedlings treated with or without 20 μM MG132 is shown. Total protein extracts from TCP14_pro:TCP14-GUS (C) and TCP15_pro:TCP15-GUS (D) were subjected to immunoblot assays using anti-GUS and anti-tubulin (as loading control) antibodies.

(E) The TCP14-GUS protein accumulates at higher levels in TCP14_pro:TCP14-GUS;da1-ko1 dar1-1 dar2-1 plants (2) than in TCP14_pro:TCP14-GUS plants (1). Total protein extracts were subjected to immunoblot assays using anti-GUS and anti-tubulin (as loading control) antibodies.

(F) The TCP15-GUS protein accumulates at higher levels in TCP15_pro:TCP15-GUS;da1-ko1 dar1-1 dar2-1 plants (2) than in TCP15_pro:TCP15-GUS plants (1). Total protein extracts were subjected to immunoblot assays using anti-GUS and anti-tubulin (as loading control) antibodies.

(G) Histochemical analysis of GUS activity in TCP14_pro:TCP14-GUS and TCP14_pro:TCP14-GUS;da1-ko1 dar1-1 dar2-1 transgenic plants measured over a period of 12 DAE.

(H) Histochemical analysis of GUS activity in TCP15_pro:TCP15-GUS and TCP15_pro:TCP15-GUS;da1-ko1 dar1-1 dar2-1 transgenic plants measured over a period of 12 DAE.

Bars in (A) and (B) = 1 cm; bars in (G) and (H) = 5 mm.