Figure 5.
TSN1 and TSN2 Are Integral Proteins of SGs and PBs.
(A) Selected time frames (0, 3, and 15 s after bleaching) from FRAP analysis of GFP-TSN1, GFP-TSN2, GFP-Rbp47b, and GFP-DCP1 in root tip cells of Col seedlings expressing ProTSN:GFP-TSN, Pro35S:GFP-Rbp47b, and Pro35S:GFP-DCP1 after heat stress (40 min at 39°C). To distinguish between SG- and PB-associated GFP-TSN1 or GFP-TSN2, both proteins were stably coexpressed with RFP-Rbp47b and RFP-DCP1, respectively. Circles and arrowheads indicate the bleached foci. Bars = 3 μm.
(B) Signal recovery rate (t1/2) of GFP fusion proteins. nd, not detected.
(C) Proportion of the initial signal recovered (%).
Data in (B) and (C) represent means ± sd of three independent experiments, each containing at least 10 seedlings.