Figure 7.

edr4-1 Affects EDR1 Focal Accumulation at the Interface between the Powdery Mildew and Arabidopsis.

(A) and (B) Focal accumulation of EDR1-GFP at the pathogen infection site in epidermal cells of 4-week-old wild-type and edr4-1 plants after inoculation with G. cichoracearum, shown in different views. Leaves were stained with propidium iodide in (B). Fungal structures are shown in red. AP, appressoria; PM, plasma membrane; PP, penetration peg; SH, secondary hyphae; SP, spores. Bars = 20 μm.

(C) Quantitative analysis of powdery mildew-induced EDR1-GFP focal accumulations (FA) in wild-type and edr4-1 mutant plants. The frequency was determined by counting the number of focal accumulations present in 25 random successful penetrations by appressoria per leaf, for three leaves per line. Bars represent means and sd of values obtained from three biological replicates per genotype. Statistically significant differences among the samples are labeled with different letters (P < 0.01, one-way ANOVA). The experiments were repeated three times with similar results.