Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 27;27(3):711–723. doi: 10.1105/tpc.114.135335

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 American Society of Plant Biologists. All rights reserved.

PMC Copyright notice

Figure 4. — Effect of the Mutation of Ser-517 of PT8 on Interaction with PHF1.

(A) Yeast split-ubiquitination assay among PHF1 and different versions of PT8. Coexpression of PHF1-Cub with NubI or NubG was used as a positive or negative control, respectively.

(B) co-IP of GFP, wild-type/mutated versions of GFP-PT8, and PHF1 from infiltrated N. benthamiana leaves. Precipitates were immunoblotted with GFP (bottom panel) and PHF1 (top panel) antibodies (Supplemental Figure 10). The first lane shows control immunoprecipitations using GFP empty vector.