Figure 1.

Organ-Specific Phenotype of See1 Demonstrating Its Role in Leaves.

(A) Disease symptoms caused by SG200∆see1 in comparison with the wild-type progenitor strain SG200 in leaves and tassels. The mutant shows a significant reduction in leaf virulence. Maize seedling leaves were scored at 12 DPI. Disease symptoms in maize tassels were scored at 14 DPI as described by Schilling et al. (2014). SG200, the virulent U. maydis progenitor strain; Δ, deletion mutant for see1; Δ/C, genetic complementation of the deletion strain. The experiment was performed in three independent biological replicates. n = number of plants infected. *P ≤ 0.001.

(B) Symptoms caused by U. maydis strain SG200 in comparison with the SG200∆see1 mutant and the complemented strain in leaves and tassels. The leaf photograph shows typical disease symptoms at 12 DPI; the tassel photograph is at 14 DPI. Similar to SG200, the mutant caused disease symptoms in tassels, but leaf tumors were significantly reduced.