Overexpression of see1 Results in Tumor Proliferation in Vegetative Tassel Parts.
(A) Tassel base abnormality occurs much more frequently with constitutive overexpression of see1 in comparison with the wild-type strain SG200. Tumor formation in the tassel base is indicated by the white arrows.
(B) Quantification of see1 gene expression in tassels infected with the overexpressing strain Ppit2-see1 in comparison with plants infected with the wild-type SG200 strain. Error bars show se. *P ≤ 0.001.
(C) Quantification of EdU-labeled tassel base cells in the in vivo DNA synthesis assay after infection with the See1-overexpressing strain Ppit2-see1 in comparison with either the wild-type SG200 infected or noninfected (N.I) tassels. There was a significant difference in the number of EdU-labeled nuclei in the abnormal tassel base region as compared with the wild-type SG200 infected or noninfected tissue. Error bars show se. Comparisons a and b, P ≤ 0.05.
(D) Detection of in vivo DNA synthesis in the tassel base colonized by See1-overexpressing strain Ppit2-see1 in comparison with tissue colonized by wild-type strain SG200 and noninfected tissue. The total nuclei were visualized by PI staining (red), and the EdU-labeled cell nuclei were visualized by AF488 staining (green). Bar = 50 μm.