Figure 5.

Overexpression of see1 Results in Tumor Proliferation in Vegetative Tassel Parts.

(A) Tassel base abnormality occurs much more frequently with constitutive overexpression of see1 in comparison with the wild-type strain SG200. Tumor formation in the tassel base is indicated by the white arrows.

(B) Quantification of see1 gene expression in tassels infected with the overexpressing strain Ppit2-see1 in comparison with plants infected with the wild-type SG200 strain. Error bars show se. *P ≤ 0.001.

(C) Quantification of EdU-labeled tassel base cells in the in vivo DNA synthesis assay after infection with the See1-overexpressing strain Ppit2-see1 in comparison with either the wild-type SG200 infected or noninfected (N.I) tassels. There was a significant difference in the number of EdU-labeled nuclei in the abnormal tassel base region as compared with the wild-type SG200 infected or noninfected tissue. Error bars show se. Comparisons a and b, P ≤ 0.05.

(D) Detection of in vivo DNA synthesis in the tassel base colonized by See1-overexpressing strain Ppit2-see1 in comparison with tissue colonized by wild-type strain SG200 and noninfected tissue. The total nuclei were visualized by PI staining (red), and the EdU-labeled cell nuclei were visualized by AF488 staining (green). Bar = 50 μm.