Expression Pattern and Subcellular Localizations of UXTs.
(A) Quantitative RT-PCR of UXT expression in Arabidopsis organs and developmental stages. The three UXTs are expressed throughout the plant with UXT1 showing the highest expression levels. Expression levels are mean ± sd (n = 3) technical replicates relative to the reference genes.
(B) Subcellular localization of the UXTs. C-terminal YFP fusions were transiently coexpressed with the α-mannosidase I Golgi marker in N. benthamiana leaves. All three UXTs colocalize with the Golgi marker. UXT1 also colocalizes with an ER marker (Supplemental Figure 1). Bar = 25 μm.